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KMID : 0614019990150010333
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Journal of Pharmaceutical Sciences (C.N.U.)
1999 Volume.15 No. 1 p.333 ~ p.338
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Determination of γ-Aminobutyric Acid and Glutamic Acid in Rat Brain by High Performance Liquid Chromatography with Electrochemical Detection
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Kang Jong-Seong
Lee Soon-Chul
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Abstract
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A sensitive and efficient assay method was applied to determine the level of glutamic acid (GA) and γ-aminobutyric acid (GABA) in frontal cortex and hippocampus of rat administrated with ethanol and drugs. The compounds were derivatized with ο-phthalaldehyde (OPA) and 2-mercaptoethanol for precolumn analysis. The condition for the simultaneous determination of GA, GABA and beta-aminobutyric acid (BABA) by high performance liquid chromatography with electrochemical detection was reverse phase C_18 column as stationary phase, 0.1M phosphate buffer containing 0.1mM Na_4EDTA: methanol=55:45 (v+v) pH 3.8 as mobile phase and 0.7V electrode voltage. The stability of reaction product of GA, GABA and BABA with OPA could be increased by adding the same volume of polyethylene glycol 400 to reaction mixture. The GABA level in frontal cortex of rat was significantly decreased by the administration of picrotoxin and diazepam, but it was significantly increased by the administration of red ginseng total saponin, N-methyl-D-glucamine and (-)-deprenyl.
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KEYWORD
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γ-Aminobutyric acid, glutamic acid, polyethylene glycol, HPLC-ECD, rat brain
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